Myself, Sambaiah Gorriparthi graduated with a Master’s degree in virology in 2012. I completed my Master degree as well as master thesis with the highest grade and distinction. Then, I worked on Pediatric HIV at the Dept. of Microbiology, Division of Clinical virology, St. John’s National Academy of Health Sciences, Bangalore, India. Later joined Indian Institute of Technology, Guwahati where I got expertise in Molecular Biology and recombinant DNA technologies like PCR, Cloning, transformation, Protein expression and purification, Crystallization of proteins. I got experience in working with viruses at Anabio Technologies, Bangalore where I handled mammalian cell lines like MDCK, HCT-8 and MRC-5, learned viral growth, viral titration by plaque assay and CCID50. Presently, I am working at Indian Immunological limited, Hyderabad as a research associate in SARS-COV2 project involved in the development of Live attenuated vaccine. I got hands on experience in viral growth in Cell culture flasks, CS-10 & Rollers, harvesting, clarification and filtration by TFF. Viral titration by plaque assay and Antibody titration of Animal studies serum sample by PRNT assay. I have Microbiology experience in Isolation, screening and culturing of microbes with various methods.
Once again, I am grateful to you for considering my application and looking forward to a favorable reply.
With profound regards
Sambaiah Gorriparthi